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KMID : 0613820120220091268
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Journal of Life Science
2012 Volume.22 No. 9 p.1268 ~ p.1276
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Molecular Epidemiology of Metallo-β-lactamase Producing Pseudomonas aeruginosa Clinical Isolates
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Choi Myung-Won
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Abstract
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The emergence and dissemination of carbapenem-resistant bacteria have resulted in limitations of antibiotic treatment and potential outbreaks of metallo-β-lactamase (MBL) producing Pseudomonas aeruginosa resistant to carbapenems. In this study, we conducted molecular characterization of the MBL genes of the β-lactam drug-resistant P. aeruginosa and prepared basic data for treatment and prevention of proliferation of antimicrobial-resistant bacterial infections. Forty-two P. aeruginosa isolates of 254 were resistant to imipenem or meropenem. Among the 42 isolates, 28 isolates were positive for the Hodge test, and 23 isolates were positive for the EDTA-disk synergy test (EDST). MBLs were detected in 59.5% (25/42) of P. aeruginosa isolates. Eight isolates harbored bla_{IMP-6}, whereas 17 isolates harbored bla_{VIM-2}. The bla_{IMP-6} gene was in a class 1 integron containing five gene cassettes: bla_{IMP-6}, qac, aacA4, bla_{OXA-1}, and aadA1. Some strains that produce IMP-6 and VIM-2 showed epidemiological relationships. The bla_{IMP-6} gene in carbapenem-resistant P. aeruginosa showed an identical pattern to a gene cassette that was reported at a hospital in Daegu, Korea. Therefore, MBL-producing P. aeruginosa is already endemic in the community. We are concerned that the existence of carbapenem-resistant bacteria containing the blaMBL gene may increase pressure on antibiotic selection when treating infections. We believe that we should select appropriate antibiotics based on the antibiotic susceptibility test and continue the research to prohibit the emergence and spread of antibiotics resistant bacteria.
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KEYWORD
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Metallo-beta-lactamase (MBL), Pseudomonas aeruginosa, bla_{VIM-2}, bla_{IMP-6}
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